Measurement

Part:BBa_K2406075:Design

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)


Lox-Term-Vox Measurement Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR primers had overhangs each containing half of the target site/terminator sequence.


Source

This part was created from round-the-horn PCR of BBa_J04450.

References